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KMID : 0378019720150080045
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New Medical Journal
1972 Volume.15 No. 8 p.45 ~ p.52
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A Study on the Determination of the Caboxyhemoglobin in Blood by the Sectrophotometer
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Abstract
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The frequent occurence of carbon monoxide poisoning necessitates the avilability of rapid and accurate methods for the determination of carboxyhemoglobin in blood samples. These methods may also be of value in evaluating the results of the usual oxygen therapy in surely poisoned patients, and in the detection of cases of chronic poisoning of moderate degree.
Here is the basis of several methods for the determination of percent saturation of hemoglobin by carbon monoxide using two-wavelength method making use of an isobestic point, which have proven to be suitable in my laboratory.
1. 0.1ml of blood is mixed with 20 ml of 0.1% ammonia solution and 10 mg. sodium dithionite is added to convert oxyhemoglobin to reduced hemoglobin. The absorbance is measured at 538nm and 578nm (isobestic point) cuvette width 1.000 cm, slit width 0.02 mm. The measurement was carried within ten minutes of addition of sodium dithionite. Absorbance measurements were made with a Hitachi Model 124 spectrophotometer and Beckman B spectrophotometer.
2. The average blood carbon monoxide content of non-smoking female was 2.01±1.45%, of male 2.30±1.05% and smoking male 3.87±2. 0%. There was no significant relation between non-smoking male and female (P>0. 01), however smoking male and non-smoking female were significant (P<0. 01).
The method given in this paper may be regarded as the most suitable spectrophotometric method for the determination of carbon monoxide saturation.
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